| First Author | Abbott C | Year | 1992 |
| Journal | Mamm Genome | Volume | 2 |
| Issue | 2 | Pages | 106-9 |
| PubMed ID | 1543905 | Mgi Jnum | J:708 |
| Mgi Id | MGI:49242 | Doi | 10.1007/BF00353858 |
| Citation | Abbott C (1992) Characterization of mouse-hamster somatic cell hybrids by PCR: a panel of mouse-specific primers for each chromosome. Mamm Genome 2(2):106-9 |
| abstractText | Mouse/hamster somatic cell hybrids form a valuable resource for mouse gene mapping. Characterization of these hybrids by isozyme analysis can be technically demanding and time-consuming. Species-specific polymerase chain reaction (PCR), where a mouse gene but not its homolog in the hamster is amplified, can provide an alternative means of characterization. Mouse-specific primers have been designed for at least one gene on each of the mouse autosomes and the X Chromosome (Chr). Primers are chosen to correspond to untranslated regions of the mouse gene concerned, in order to decrease the chance of cross-hybridization with the homologous hamster gene. These primer sequences are presented, together with the conditions for their use. |
