Primary Identifier | MGI:4868693 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Cflar |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted just upstream of exon 2 (containing the ATG start codon). A floxed neomycin resistance cassette was also inserted in intron 3. The resistance cassette was removed prior to blastocyst injection, presumably by transient cre excision. This left exons 2 and 3 flanked by loxP sites. Miced with this floxed allele were then crossed to mice carrying a Zp3-cre transgene to produce the deleted allele. |