First Author | Maves KK | Year | 1995 |
Journal | Immunology | Volume | 86 |
Issue | 3 | Pages | 475-9 |
PubMed ID | 8550088 | Mgi Jnum | J:29699 |
Mgi Id | MGI:77224 | Citation | Maves KK, et al. (1995) Cloning and characterization of the cDNA encoding guinea-pig properdin: a comparison of properdin from three species. Immunology 86(3):475-9 |
abstractText | The cDNA sequence encoding properdin was generated from guinea-pig spleen RNA by the reverse transcription-polymerase chain reaction. This sequence was approximately 75% homologous with human and 71% homologous with murine properdin at the nucleic acid level. Guinea-pig properdin had six thrombospondin repeat sequences consisting of about 60 amino acids, each with six cysteine and three tryptophan residues. Additionally, the Valine-Threonine-Cysteine-Glycine sequence, reported to have important cell adhesive properties in malarial circumsporozoite proteins and thrombospondin, was conserved in the properdin sequence of guinea-pigs. Finally, mouse spleen was also examined to complete the sequence determination of the leader peptide and the initial four residues of murine properdin. This allowed a thorough comparison of the primary structure of properdin from all three species. Like human and murine properdin cDNAs, the guinea pig sequence contained a region of unique, non-homologous sequence (18 base pairs in length) within the fifth thrombospondin repeat, the significance of which remains unclear. |