| First Author | Liu S | Year | 2002 |
| Journal | Development | Volume | 129 |
| Issue | 24 | Pages | 5839-46 |
| PubMed ID | 12421721 | Mgi Jnum | J:79896 |
| Mgi Id | MGI:2389540 | Doi | 10.1242/dev.00173 |
| Citation | Liu S, et al. (2002) A defect in a novel Nek-family kinase causes cystic kidney disease in the mouse and in zebrafish. Development 129(24):5839-46 |
| abstractText | The murine autosomal recessive juvenile cystic kidney (jck) mutation results in polycystic kidney disease. We have identified in jck mice a mutation in Nek8, a novel and highly conserved member of the Nek kinase family. In vitro expression of mutated Nek8 results in enlarged, multinucleated cells with an abnormal actin cytoskeleton. To confirm that a defect in the Nek8 gene can cause cystic disease, we performed a cross-species analysis: injection of zebrafish embryos with a morpholino anti-sense oligonucleotide corresponding to the ortholog of Nek8 resulted in the formation of pronephric cysts. These results demonstrate that comparative analysis of gene function in different model systems represents a powerful means to annotate gene function. |
