Primary Identifier | MGI:4432238 | Allele Type | Targeted |
Attribute String | Conditional ready, Hypomorph, Null/knockout, Reporter | Gene | Cenpj |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
Project Collection | EUCOMM |
description | Special note about mutant cell line EPD0028_7_G05: The mutation generated in this ES cell line results in a hypomorphic allele. A low but detectable amount of splicing over the gene-trap cassette was observed in MEFs derived from mutant mice (2.16% of wild-type exon 4-5 levels); immunoblotting confirmed expression of low levels of apparently full-length encoded protein. |
molecularNote | The L1L2_gt1 cassette was inserted at position 56792283 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 56793168. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |