| First Author | Rudolf A | Year | 2016 |
| Journal | Cell Rep | Volume | 15 |
| Issue | 6 | Pages | 1277-90 |
| PubMed ID | 27134174 | Mgi Jnum | J:235478 |
| Mgi Id | MGI:5796449 | Doi | 10.1016/j.celrep.2016.04.022 |
| Citation | Rudolf A, et al. (2016) beta-Catenin Activation in Muscle Progenitor Cells Regulates Tissue Repair. Cell Rep 15(6):1277-90 |
| abstractText | Skeletal muscle regeneration relies on a pool of resident muscle stem cells called satellite cells (MuSCs). Following injury-induced destruction of the myofibers, quiescent MuSCs are activated and generate transient amplifying progenitors (myoblasts) that will fuse to form new myofibers. Here, we focus on the canonical Wnt signaling pathway and find that either conditional beta-catenin disruption or activation in adult MuSCs results in perturbation of muscle regeneration. Using both in vivo and in vitro approaches, we observed that myoblasts lacking beta-catenin show delayed differentiation, whereas myoblasts with constitutively active beta-catenin undergo precocious growth arrest and differentiation. Transcriptome analysis further demonstrated that Wnt/beta-catenin signaling interacts with multiple pathways and, more specifically, TGF-beta signaling. Indeed, exogenous TGF-beta2 stimulation restores the regenerative potential of muscles with targeted beta-catenin disruption in MuSCs. We conclude that a precise level of beta-catenin activity is essential for regulating the amplification and differentiation of MuSC descendants during adult myogenesis. |
