Primary Identifier | MGI:6357845 | Allele Type | Targeted |
Attribute String | Recombinase, Reporter | Gene | Klrg1 |
Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
Is Recombinase | true | Is Wild Type | false |
molecularNote | The targeting construct containing a Frt-flanked Neomycin resistance gene (Neo), a thymidine kinase, and a fragment encoding an IRES-eGFP-iCre fusion protein was used for homologous recombination in ES cells to insert the reporter cassette immediately after the stop codon of the Klrg1 gene. Germline transmitted mice were bred with FLPo mice to remove the neo gene. |