| First Author | Abulizi A | Year | 2019 |
| Journal | FASEB J | Volume | 33 |
| Issue | 7 | Pages | 8174-8185 |
| PubMed ID | 30922125 | Mgi Jnum | J:292624 |
| Mgi Id | MGI:6448548 | Doi | 10.1096/fj.201802706R |
| Citation | Abulizi A, et al. (2019) Adipose glucocorticoid action influences whole-body metabolism via modulation of hepatic insulin action. FASEB J 33(7):8174-8185 |
| abstractText | The connection between adipose glucocorticoid action and whole-body metabolism is incompletely understood. Thus, we generated adipose tissue-specific glucocorticoid receptor-knockout (Ad-GcR(-/-)) mice to explore potential mechanisms. Ad-GcR(-/-) mice had a lower concentration of fasting plasma nonesterified fatty acids and less hepatic steatosis. This was associated with increased protein kinase B phosphorylation and increased hepatic glycogen synthesis after an oral glucose challenge. High-fat diet (HFD)-fed Ad-GcR(-/-) mice were protected against the development of hepatic steatosis and diacylglycerol-PKCepsilon-induced impairments in hepatic insulin signaling. Under hyperinsulinemic-euglycemic conditions, hepatic insulin response was approximately 10-fold higher in HFD-fed Ad-GcR(-/-) mice. Insulin-mediated suppression of adipose lipolysis was improved by 40% in Ad-GcR(-/-) mice. Adipose triglyceride lipase expression was decreased and insulin-mediated perilipin dephosphorylation was increased in Ad-GcR(-/-) mice. In metabolic cages, food intake decreased by 3 kcal/kg per hour in Ad-GcR(-/-) mice. Therefore, physiologic adipose glucocorticoid action appears to drive hepatic lipid accumulation during stressors such as fasting. The resultant hepatic insulin resistance prevents hepatic glycogen synthesis, preserving glucose for glucose-dependent organs. Absence of adipose glucocorticoid action attenuates HFD-induced hepatic insulin resistance; potential explanations for reduction in hepatic steatosis include reductions in adipose lipolysis and food intake.-Abulizi, A., Camporez, J.-P., Jurczak, M. J., Hoyer, K. F., Zhang, D., Cline, G. W., Samuel, V. T., Shulman, G. I., Vatner, D. F. Adipose glucocorticoid action influences whole-body metabolism via modulation of hepatic insulin action. |
