Primary Identifier | MGI:7645616 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Marveld3 |
Strain of Origin | C57BL/6 x DBA/2 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/Cas9 technology using crRNA 5â-TGTCGCCCTCGGATCGTTTC-3â and crRNA 5â-GGACCAGGGGACCGCCGAGGG-3â deleted approximately 686 bp, encompassing the transcription initiation site, the first exon and part of the first intron. While RT-PCR showed 90% reduction in the expression of both isoform-coding mRNAs in E15.5 pancreas, Western blot analysis showed absence of protein in adult pancreatic and renal extracts. |