| First Author | Schuh K | Year | 2004 |
| Journal | J Biol Chem | Volume | 279 |
| Issue | 27 | Pages | 28220-6 |
| PubMed ID | 15078889 | Mgi Jnum | J:121031 |
| Mgi Id | MGI:3709130 | Doi | 10.1074/jbc.M312599200 |
| Citation | Schuh K, et al. (2004) Plasma membrane Ca2+ ATPase 4 is required for sperm motility and male fertility. J Biol Chem 279(27):28220-6 |
| abstractText | Calcium and Ca(2+)-dependent signals play a crucial role in sperm motility and mammalian fertilization, but the molecules and mechanisms underlying these Ca(2+)-dependent pathways are incompletely understood. Here we show that homozygous male mice with a targeted gene deletion of isoform 4 of the plasma membrane calcium/calmodulin-dependent calcium ATPase (PMCA), which is highly enriched in the sperm tail, are infertile due to severely impaired sperm motility. Furthermore, the PMCA inhibitor 5-(and-6)-carboxyeosin diacetate succinimidyl ester reduced sperm motility in wild-type animals, thus mimicking the effects of PMCA4 deficiency on sperm motility and supporting the hypothesis of a pivotal role of the PMCA4 on the regulation of sperm function and intracellular Ca(2+) levels. |
