| First Author | Liu Y | Year | 1993 |
| Journal | Mol Cell Biol | Volume | 13 |
| Issue | 3 | Pages | 1836-46 |
| PubMed ID | 8441416 | Mgi Jnum | J:3999 |
| Mgi Id | MGI:52504 | Doi | 10.1128/mcb.13.3.1836 |
| Citation | Liu Y, et al. (1993) COUP-TF acts as a competitive repressor for estrogen receptor-mediated activation of the mouse lactoferrin gene. Mol Cell Biol 13(3):1836-46 |
| abstractText | We previously demonstrated that the estrogen response module (mERM) of the mouse lactoferrin gene, which contains an overlapping chicken ovalbumin upstream promoter transcription factor (COUP-TF)- and estrogen receptor-binding element, is responsible for estrogen induction. In this report we show that COUP-TF represses the mERM response to estrogen stimulation. Mutation and deletion of the COUP-TF-binding element or reduction of the endogenous COUP-TF increases mERM estrogen responsiveness. Likewise, overexpression of the COUP-TF expression vector blocked the estrogen-stimulated response of mERM in transfected cells. The molecular mechanism of this repression is due to the competition between COUP-TF and the estrogen receptor for binding at identical contact sites in the overlapping region of the mERM. Our results indicate that two members of the steroid-thyroid receptor superfamily work in concert to modulate lactoferrin gene expression. |
