Primary Identifier | MGI:4887402 | Allele Type | Targeted |
Gene | Gnas | Transmission | Germline |
Strain of Origin | 129S/SvEv | Is Recombinase | false |
Is Wild Type | false |
description | ES cell = MAC3. |
molecularNote | A rabbit beta-globin polyadenylation cassette was inserted near the end of the exon 1A to truncate the transcript upon paternal transmission. A loxP flanked neomycin cre cassette was inserted immediately downstream as well. RT-PCR and Northern blot analysis confirmed that reduced amounts of a trucated mRNA were formed. |