| First Author | LeVine AM | Year | 1999 |
| Journal | J Clin Invest | Volume | 103 |
| Issue | 7 | Pages | 1015-21 |
| PubMed ID | 10194474 | Mgi Jnum | J:110384 |
| Mgi Id | MGI:3640139 | Doi | 10.1172/JCI5849 |
| Citation | LeVine AM, et al. (1999) Surfactant protein-A enhances respiratory syncytial virus clearance in vivo. J Clin Invest 103(7):1015-21 |
| abstractText | To determine the role of surfactant protein-A(SP-A) in antiviral host defense, mice lacking SP-A (SP-A-/-) were produced by targeted gene inactivation. SP-A-/- and control mice (SP-A+/+) were infected with respiratory syncytial virus (RSV) by intratracheal instillation. Pulmonary infiltration after infection was more severe in SP-A-/- than in SP-A+/+ mice and was associated with increased RSV plaque-forming units in lung homogenates. Pulmonary infiltration with polymorphonuclear leukocytes was greater in the SP-A-/- mice. Levels of proinflammatory cytokines tumor necrosis factor-alpha and interleukin-6 were enhanced in lungs of SP-A-/- mice. After RSV infection, superoxide and hydrogen peroxide generation was deficient in macrophages from SP-A-/- mice, demonstrating a critical role of SP-A in oxidant production associated with RSV infection. Coadministration of RSV with exogenous SP-A reduced viral titers and inflammatory cells in the lung of SP-A-/- mice. These findings demonstrate that SP-A plays an important host defense role against RSV in vivo. |
