| First Author | Silva S | Year | 2017 |
| Journal | RNA | Volume | 23 |
| Issue | 9 | Pages | 1385-1392 |
| PubMed ID | 28539487 | Mgi Jnum | J:243740 |
| Mgi Id | MGI:5911541 | Doi | 10.1261/rna.060723.117 |
| Citation | Silva S, et al. (2017) Characterization of the mammalian RNA exonuclease 5/NEF-sp as a testis-specific nuclear 3' --> 5' exoribonuclease. RNA 23(9):1385-1392 |
| abstractText | Ribonucleases catalyze maturation of functional RNAs or mediate degradation of cellular transcripts, activities that are critical for gene expression control. Here we identify a previously uncharacterized mammalian nuclease family member NEF-sp (RNA exonuclease 5 [REXO5] or LOC81691) as a testis-specific factor. Recombinant human NEF-sp demonstrates a divalent metal ion-dependent 3' --> 5' exoribonuclease activity. This activity is specific to single-stranded RNA substrates and is independent of their length. The presence of a 2'-O-methyl modification at the 3' end of the RNA substrate is inhibitory. Ectopically expressed NEF-sp localizes to the nucleolar/nuclear compartment in mammalian cell cultures and this is dependent on an amino-terminal nuclear localization signal. Finally, mice lacking NEF-sp are viable and display normal fertility, likely indicating overlapping functions with other nucleases. Taken together, our study provides the first biochemical and genetic exploration of the role of the NEF-sp exoribonuclease in the mammalian genome. |
