First Author | Tanoue T | Year | 2004 |
Journal | J Cell Biol | Volume | 165 |
Issue | 4 | Pages | 517-28 |
PubMed ID | 15148305 | Mgi Jnum | J:91137 |
Mgi Id | MGI:3046012 | Doi | 10.1083/jcb.200403006 |
Citation | Tanoue T, et al. (2004) Mammalian Fat1 cadherin regulates actin dynamics and cell-cell contact. J Cell Biol 165(4):517-28 |
abstractText | Fat cadherins form a distinct subfamily of the cadherin gene superfamily, and are featured by their unusually large extracellular domain. In this work, we investigated the function of a mammalian Fat cadherin. Fat1 was localized at filopodial tips, lamellipodial edges, and cell-cell boundaries, overlapping with dynamic actin structures. RNA interference-mediated knockdown of Fat1 resulted in disorganization of cell junction-associated F-actin and other actin fibers/cables, disturbance of cell-cell contacts, and also inhibition of cell polarity formation at wound margins. Furthermore, we identified Ena/vasodilator-stimulated phosphoproteins as a potential downstream effector of Fat1. These results suggest that Fat1 regulates actin cytoskeletal organization at cell peripheries, thereby modulating cell contacts and polarity. |