First Author | Kneppers A | Year | 2023 |
Journal | iScience | Volume | 26 |
Issue | 12 | Pages | 108343 |
PubMed ID | 38077152 | Mgi Jnum | J:343732 |
Mgi Id | MGI:7566908 | Doi | 10.1016/j.isci.2023.108343 |
Citation | Kneppers A, et al. (2023) AMPKalpha2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretion. iScience 26(12):108343 |
abstractText | Due to the post-mitotic nature of skeletal muscle fibers, adult muscle maintenance relies on dedicated muscle stem cells (MuSCs). In most physiological contexts, MuSCs support myofiber homeostasis by contributing to myonuclear accretion, which requires a coordination of cell-type specific events between the myofiber and MuSCs. Here, we addressed the role of the kinase AMPKalpha2 in the coordination of these events supporting myonuclear accretion. We demonstrate that AMPKalpha2 deletion impairs skeletal muscle regeneration. Through in vitro assessments of MuSC myogenic fate and EdU-based cell tracing, we reveal a MuSC-specific role of AMPKalpha2 in the regulation of myonuclear accretion, which is mediated by phosphorylation of the non-metabolic substrate BAIAP2. Similar cell tracing in vivo shows that AMPKalpha2 knockout mice have a lower rate of myonuclear accretion during regeneration, and that MuSC-specific AMPKalpha2 deletion decreases myonuclear accretion in response to myofiber contraction. Together, this demonstrates that AMPKalpha2 is a MuSC-intrinsic regulator of myonuclear accretion. |