Primary Identifier | MGI:3046841 | Allele Type | Targeted |
Attribute String | Modified regulatory region, Null/knockout | Gene | Otx2 |
Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The anterior neuroectoderm (AN) enhancer region (559 bp) was replaced with a floxed neomycin-resistance gene. Southern blot confirmed recombination and quantitative RT-PCR confirmed a reduction in gene expression in mutant embryos. |