| First Author | García-Tuñón I | Year | 2020 |
| Journal | Mol Biol Rep | Volume | 47 |
| Issue | 2 | Pages | 1381-1391 |
| PubMed ID | 31833031 | Mgi Jnum | J:287300 |
| Mgi Id | MGI:6405635 | Doi | 10.1007/s11033-019-05214-7 |
| Citation | Garcia-Tunon I, et al. (2020) Establishment of a conditional Nomo1 mouse model by CRISPR/Cas9 technology. Mol Biol Rep 47(2):1381-1391 |
| abstractText | The Nomo1 gene mediates a wide range of biological processes of importance in embryonic development. Accordingly, constitutive perturbation of Nomo1 function may result in myriad developmental defects that trigger embryonic lethality. To extend our understanding of Nomo1 function in postnatal stages and in a tissue-specific manner, we generated a conditional knockout mouse model of Nomo1. To achieve this, we used clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology in C57Bl/6J mouse zygotes to generate a new mouse model in which exon 3 of the Nomo1 gene is specifically flanked (or floxed) by LoxP sites (Nomo1(f/f)). Nomo1(f/f) mouse embryonic fibroblasts were transduced with a Cre adenovirus and efficiently recombined between LoxP sites. Genomic and expression studies in Nomo1-transduced MEFs demonstrated that the Nomo1 exon 3 is ablated. Western blot assay showed that no protein or early truncated protein is produced. In vivo assay crossing Nomo1(f/f) mouse with a Msi1-CRE transgenic mouse corroborated the previous findings and it showed Nomo1 exon 3 deletion at msi1+ cell compartment. This short technical report demonstrates that CRISPR/Cas9 technology is a simple and easy method for creating conditional mouse models. The Nomo1(f/f) mouse will be useful to researchers who wish to explore the role of Nomo1 in any developmental stage or in a tissue-specific manner. |
