| First Author | Nguyen MT | Year | 2019 |
| Journal | Nat Cell Biol | Volume | 21 |
| Issue | 4 | Pages | 420-429 |
| PubMed ID | 30936473 | Mgi Jnum | J:279052 |
| Mgi Id | MGI:6359287 | Doi | 10.1038/s41556-019-0301-x |
| Citation | Nguyen MT, et al. (2019) An opsin 5-dopamine pathway mediates light-dependent vascular development in the eye. Nat Cell Biol 21(4):420-429 |
| abstractText | During mouse postnatal eye development, the embryonic hyaloid vascular network regresses from the vitreous as an adaption for high-acuity vision. This process occurs with precisely controlled timing. Here, we show that opsin 5 (OPN5; also known as neuropsin)-dependent retinal light responses regulate vascular development in the postnatal eye. In Opn5-null mice, hyaloid vessels regress precociously. We demonstrate that 380-nm light stimulation via OPN5 and VGAT (the vesicular GABA/glycine transporter) in retinal ganglion cells enhances the activity of inner retinal DAT (also known as SLC6A3; a dopamine reuptake transporter) and thus suppresses vitreal dopamine. In turn, dopamine acts directly on hyaloid vascular endothelial cells to suppress the activity of vascular endothelial growth factor receptor 2 (VEGFR2) and promote hyaloid vessel regression. With OPN5 loss of function, the vitreous dopamine level is elevated and results in premature hyaloid regression. These investigations identify violet light as a developmental timing cue that, via an OPN5-dopamine pathway, regulates optic axis clearance in preparation for visual function. |
