| First Author | Lai CW | Year | 2013 |
| Journal | J Neurosci | Volume | 33 |
| Issue | 18 | Pages | 7941-51 |
| PubMed ID | 23637185 | Mgi Jnum | J:197148 |
| Mgi Id | MGI:5490935 | Doi | 10.1523/JNEUROSCI.5001-12.2013 |
| Citation | Lai CW, et al. (2013) Phosducin-like protein 1 is essential for G-protein assembly and signaling in retinal rod photoreceptors. J Neurosci 33(18):7941-51 |
| abstractText | G-protein beta subunits perform essential neuronal functions as part of G-protein betagamma and Gbeta5-regulators of G-protein signaling (RGS) complexes. Both Gbetagamma and Gbeta5-RGS are obligate dimers that are thought to require the assistance of the cytosolic chaperonin CCT and a cochaperone, phosducin-like protein 1 (PhLP1) for dimer formation. To test this hypothesis in vivo, we deleted the Phlp1 gene in mouse (Mus musculus) retinal rod photoreceptor cells and measured the effects on G-protein biogenesis and visual signal transduction. In the PhLP1-depleted rods, Gbetagamma dimer formation was decreased 50-fold, resulting in a >10-fold decrease in light sensitivity. Moreover, a 20-fold reduction in Gbeta5 and RGS9-1 expression was also observed, causing a 15-fold delay in the shutoff of light responses. These findings conclusively demonstrate in vivo that PhLP1 is required for the folding and assembly of both Gbetagamma and Gbeta5-RGS9. |
