| First Author | Lam KP | Year | 1999 |
| Journal | J Exp Med | Volume | 190 |
| Issue | 4 | Pages | 471-7 |
| PubMed ID | 10449518 | Mgi Jnum | J:115292 |
| Mgi Id | MGI:3691356 | Doi | 10.1084/jem.190.4.471 |
| Citation | Lam KP, et al. (1999) B cell antigen receptor specificity and surface density together determine B-1 versus B-2 cell development. J Exp Med 190(4):471-7 |
| abstractText | Mice expressing the immunoglobulin (Ig) heavy (H) chain variable (V) region from a rearranged V(H)12 gene inserted into the IgH locus generate predominantly B-1 cells, whereas expression of two other V(H) region transgenes (V(H)B1-8 and V(H)glD42) leads to the almost exclusive generation of conventional, or B-2, cells. To determine the developmental potential of B cells bearing two distinct B cell antigen receptors (BCRs), one favoring B-1 and the other favoring B-2 cell development, we crossed V(H)12 insertion mice with mice bearing either V(H)B1-8 or V(H)glD42. B cells coexpressing V(H)12 and one of the other V(H) genes are readily detected in the double IgH insertion mice, and are of the B-2 phenotype. In mice coexpressing V(H)12, V(H)B1-8 and a transgenic kappa chain able to pair with both H chains, double H chain-expressing B-2 cells, and B-1 cells that have lost V(H)B1-8 are generated, whereas V(H)B1-8 single producers are undetectable. These data suggest that B-1 but not B-2 cells are selected by antigenic stimuli in whose delivery BCR specificity and surface density are of critical importance. |
