| First Author | Cooper C | Year | 1992 |
| Journal | J Immunol | Volume | 149 |
| Issue | 10 | Pages | 3225-31 |
| PubMed ID | 1431100 | Mgi Jnum | J:113698 |
| Mgi Id | MGI:3687437 | Doi | 10.4049/jimmunol.149.10.3225 |
| Citation | Cooper C, et al. (1992) The C/EBP family of transcriptional activators is functionally important for Ig VH promoter activity in vivo and in vitro. J Immunol 149(10):3225-31 |
| abstractText | We have examined the functional importance of binding sites for C/EBP family members (E sites), in two Ig VH promoters: VH1, a member of the S107 family, and BCL1, a member of the J558 family. Mutation of the E site in the VH1 promoter diminishes transcription in vivo to 59% of wild-type and transcription from the BCL1 promoter in vitro is inhibited to an average of 39% of wild-type by competition with E site oligonucleotides. Purified E site binding proteins from plasmacytoma cells stimulated BCL1 transcription in vitro 2.3-fold. Although five C/EBP family proteins are known which bind the E site, antibody ablation of DNA:protein complexes resolved by electrophoretic mobility shift assays showed that Ig/EBP-1 is the only E site binding protein detectable in early B cell lines; more mature B cells contain Ig/EBP-1 and NF-IL6. We also show by antibody-depletion that Ig/EBP-1 activates the BCL1 promoter in vitro. |
