| First Author | Wickham L | Year | 2005 |
| Journal | J Neurochem | Volume | 92 |
| Issue | 1 | Pages | 93-102 |
| PubMed ID | 15606899 | Mgi Jnum | J:95653 |
| Mgi Id | MGI:3526749 | Doi | 10.1111/j.1471-4159.2004.02840.x |
| Citation | Wickham L, et al. (2005) Beta-amyloid protein converting enzyme 1 and brain-specific type II membrane protein BRI3: binding partners processed by furin. J Neurochem 92(1):93-102 |
| abstractText | Using a yeast two-hybrid system, we screened a human brain cDNA library for possible interacting proteins with the C-terminal cytosolic tail of the beta-secretase beta-amyloid protein converting enzyme (BACE)1. This identified seven potential candidates, including the brain-specific type II membrane protein BRI3. Co-localization and co-immunoprecipitation experiments confirmed that BACE1 and BRI3 co-localize and interact with each other via the cytosolic tail of BACE1. Furthermore, pulse and pulse-chase analyses revealed that the pro-protein convertases furin, and to a lesser extent PC7 and PC5A, process BRI3 into a C-terminal secreted approximately 4-kDa product. Thus, furin efficiently processes both pro-BACE1 and its novel interacting protein pro-BRI3. |
