| First Author | Zwilling S | Year | 1997 |
| Journal | Science | Volume | 277 |
| Issue | 5323 | Pages | 221-5 |
| PubMed ID | 9211847 | Mgi Jnum | J:41470 |
| Mgi Id | MGI:893948 | Doi | 10.1126/science.277.5323.221 |
| Citation | Zwilling S, et al. (1997) Inducible expression and phosphorylation of coactivator BOB.1/OBF.1 in T cells [see comments]. Science 277(5323):221-5 |
| abstractText | BOB.1/OBF.1 is a transcriptional coactivator that is constitutively expressed in B cells and interacts with the Oct1 and Oct2 transcription factors. Upon activation of Jurkat T cells and primary murine thymocytes with phorbol esters and ionomycin, BOB.1/OBF.1 expression and transactivation function were induced. BOB.1/OBF.1 was phosphorylated at Ser184 both in vivo and in vitro, and this modification was required for inducible activation. Mutation of Ser184 also diminished transactivation function in B cells, suggesting that the activating phosphorylation that is inducible in T cells is constitutively present in B cells. Thus, BOB.1/OBF.1 is a transcriptional coactivator that is critically regulated by posttranslational modifications to mediate cell type-specific gene expression. |
