| Primary Identifier | MGI:7386905 | Allele Type | Targeted |
| Attribute String | Modified regulatory region | Gene | Del(14Rr299391-Rr308)3Fwa |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 1085 bp region containing Tcrd enhancer Edelta was replaced with a loxP site flanked neomycin resistance gene cassette, which was subsequently removed via cre-mediated recombination. A 1 kb PvuII-BamHI genomic fragment containing Tcra enhancer Ealpha (Rr308) was replaced with a loxP site flanked neomycin resistance gene cassette. The neo cassette was subsequently deleted through cre-mediated recombination. Further cre-mediated recombination between the single loxP sites left in place of Edelta and in place of Ealpha results in the deletion of Trdc, Trdv5, the entire Traj cluster and Trac. |
