| First Author | Tarabykina S | Year | 2000 |
| Journal | FEBS Lett | Volume | 475 |
| Issue | 3 | Pages | 187-91 |
| PubMed ID | 10869553 | Mgi Jnum | J:127191 |
| Mgi Id | MGI:3763311 | Doi | 10.1016/s0014-5793(00)01652-5 |
| Citation | Tarabykina S, et al. (2000) Heterocomplex formation between metastasis-related protein S100A4 (Mts1) and S100A1 as revealed by the yeast two-hybrid system. FEBS Lett 475(3):187-91 |
| abstractText | S100A4 (Mts1) is a Ca(2+)-binding protein of the S100 family. This protein plays an important role in promoting tumor metastasis. In order to identify S100A4 interacting proteins, we have applied the yeast two-hybrid system as an in vivo approach. By screening a mouse mammary adenocarcinoma library, we have demonstrated that S100A4 forms a heterocomplex with S100A1, another member of the S100 family. The non-covalent heterodimerization was confirmed by fluorescence spectroscopy and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. Mutational analysis revealed that replacement of Cys(76) and/or Cys(81) of S100A4 by Ser abolishes the S100A4/S100A1 heterodimerization, but does not affect the S100A4 homodimerization in vivo. |
