| Primary Identifier | MGI:7620301 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Eed |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129P2/OlaHsd)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting construct inserted a loxP upstream of the cDNA encoding exon 6 through 10 followed by the 3'UTR, six polyadenylation sequences, and loxP site upstream of exon 6. Additionally, a GFP reporter gene was inserted at the end of exon 6 followed by a polyadenylation sequence. Flp-mediated recombination removed the self-excising, FRT-flanked selection cassette. Cre-mediated recombination removed the cDNA encoding exons 6 through 10, allowing expression of the GFP reporter gene. |
