Primary Identifier | MGI:2135519 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Pdgfrb |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A promoterless beta-galactosidase/neomycin gene fusion (beta-geo) was inserted in frame into the exon coding for the signal peptide. Southern blot analysis demonstrated that three tandem copies of the beta-geo gene were present in a concatamer. Northern blot analysis failed to detect a PDGFbetaR transcript in homozygous mutants. Western blot analysis of total protein failed to detect any immunoreactive PDGFbetaR in homozygous mutants. |