| First Author | Nandadasa S | Year | 2021 |
| Journal | Matrix Biol | Volume | 97 |
| Pages | 40-57 | PubMed ID | 33454424 |
| Mgi Jnum | J:306231 | Mgi Id | MGI:6709851 |
| Doi | 10.1016/j.matbio.2021.01.002 | Citation | Nandadasa S, et al. (2021) The versican-hyaluronan complex provides an essential extracellular matrix niche for Flk1(+) hematoendothelial progenitors. Matrix Biol 97:40-57 |
| abstractText | Little is known about extracellular matrix (ECM) contributions to formation of the earliest cell lineages in the embryo. Here, we show that the proteoglycan versican and glycosaminoglycan hyaluronan are associated with emerging Flk1(+) hematoendothelial progenitors at gastrulation. The mouse versican mutant Vcan(hdf) lacks yolk sac vasculature, with attenuated yolk sac hematopoiesis. CRISPR/Cas9-mediated Vcan inactivation in mouse embryonic stem cells reduced vascular endothelial and hematopoietic differentiation within embryoid bodies, which generated fewer blood colonies, and had an impaired angiogenic response to VEGF165. Hyaluronan was severely depleted in Vcan(hdf) embryos, with corresponding upregulation of the hyaluronan-depolymerase TMEM2. Conversely, hyaluronan-deficient mouse embryos also had vasculogenic suppression but with increased versican proteolysis. VEGF165 and Indian hedgehog, crucial vasculogenic factors, utilized the versican-hyaluronan matrix, specifically versican chondroitin sulfate chains, for binding. Versican-hyaluronan ECM is thus an obligate requirement for vasculogenesis and primitive hematopoiesis, providing a vasculogenic factor-enriching microniche for Flk1(+) progenitors from their origin at gastrulation. |
