| First Author | Chang J | Year | 2023 |
| Journal | Front Immunol | Volume | 14 |
| Pages | 1239779 | PubMed ID | 37662955 |
| Mgi Jnum | J:340321 | Mgi Id | MGI:7527330 |
| Doi | 10.3389/fimmu.2023.1239779 | Citation | Chang J, et al. (2023) Impaired tissue homing by the Ikzf3(N159S) variant is mediated by interfering with Ikaros function. Front Immunol 14:1239779 |
| abstractText | AIOLOS, encoded by IKZF3, is a member of the IKZF family of proteins that plays an important role in regulating late B-cell differentiation. Human individuals heterozygous for the AIOLOS p.N160S variant displayed impaired humoral immune responses as well as impaired B and T cell development. We have previously reported that a mouse strain harboring an Ikzf3(N159S) allele that corresponds to human IKZF3(N160S) recapitulated immune-deficient phenotypes, such as impaired B cell development and loss of CD23 expression. In this study, we investigated the effect of the Ikzf3(N159S) variant and found that B1a cell development was impaired in Ikzf3(N159S/N159S) mice. In addition, CD62L expression was severely decreased in both B and T lymphocytes by the Ikzf3(N159S) mutation, in a dose-dependent manner. Mixed bone marrow chimera experiments have revealed that most immunodeficient phenotypes, including low CD62L expression, occur in intrinsic cells. Interestingly, while Ikzf3(N159S/N159S) lymphocytes were still present in the spleen, they were completely outcompeted by control cells in the lymph nodes, suggesting that the capacity for homing or retention in the lymph nodes was lost due to the Ikzf3(N159S) mutation. The homing assay confirmed severely decreased homing abilities to lymph nodes of Ikzf3(N159S/N159S) B and T lymphocytes but selective enrichment of CD62L expressing Ikzf3(N159S/N159S) lymphocytes in lymph nodes. This finding suggests that impaired CD62L expression is the major reason for the impaired homing capacity caused by the Ikzf3(N159S) mutation. Interestingly, an excess amount of Ikaros, but not Aiolos, restored CD62L expression in Ikzf3(N159S/N159S) B cells. Together with the loss of CD62L expression due to Ikaros deficiency, the Aiolos(N159S) mutant protein likely interferes with Ikaros function through heterodimerization, at least in activating the Sell gene encoding CD62L expression. Thus, our results revealed that Aiolos(N159S) causes some immunodeficient phenotypes via the pathogenesis referred to as the heterodimeric interference as observed for Aiolos(G158R) variant. |
