| Primary Identifier | MGI:2445310 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Plcb3 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 4 kb genomic fragment encompassing exons 11 through 17 was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region (encoding amino acids 338 through 681) included portions of the X and Y domains, which are putatively essential for enzymatic activity, and a nuclear targeting signal. Western blot analysis showed protein levels to be reduced by approximately 50% in lung tissue obtained from heterozygous mutant mice. |
