| First Author | Sebag SC | Year | 2021 |
| Journal | Cell Rep | Volume | 37 |
| Issue | 7 | Pages | 110003 |
| PubMed ID | 34788615 | Mgi Jnum | J:328317 |
| Mgi Id | MGI:6881870 | Doi | 10.1016/j.celrep.2021.110003 |
| Citation | Sebag SC, et al. (2021) ADH5-mediated NO bioactivity maintains metabolic homeostasis in brown adipose tissue. Cell Rep 37(7):110003 |
| abstractText | Brown adipose tissue (BAT) thermogenic activity is tightly regulated by cellular redox status, but the underlying molecular mechanisms are incompletely understood. Protein S-nitrosylation, the nitric-oxide-mediated cysteine thiol protein modification, plays important roles in cellular redox regulation. Here we show that diet-induced obesity (DIO) and acute cold exposure elevate BAT protein S-nitrosylation, including UCP1. This thermogenic-induced nitric oxide bioactivity is regulated by S-nitrosoglutathione reductase (GSNOR; alcohol dehydrogenase 5 [ADH5]), a denitrosylase that balances the intracellular nitroso-redox status. Loss of ADH5 in BAT impairs cold-induced UCP1-dependent thermogenesis and worsens obesity-associated metabolic dysfunction. Mechanistically, we demonstrate that Adh5 expression is induced by the transcription factor heat shock factor 1 (HSF1), and administration of an HSF1 activator to BAT of DIO mice increases Adh5 expression and significantly improves UCP1-mediated respiration. Together, these data indicate that ADH5 controls BAT nitroso-redox homeostasis to regulate adipose thermogenesis, which may be therapeutically targeted to improve metabolic health. |
