| First Author | Waters SH | Year | 1992 |
| Journal | Diabetes | Volume | 41 |
| Issue | 3 | Pages | 308-12 |
| PubMed ID | 1372574 | Mgi Jnum | J:1986 |
| Mgi Id | MGI:50510 | Doi | 10.2337/diab.41.3.308 |
| Citation | Waters SH, et al. (1992) Multiple TCR V beta usage by infiltrates of young NOD mouse islets of Langerhans. A polymerase chain reaction analysis. Diabetes 41(3):308-12 |
| abstractText | Because a restricted repertoire of T-cell receptor (TCR) V beta gene expression has been reported in other autoimmune diseases, the possibility of similarly restricted V beta gene expression by T-cell infiltrates of NOD mouse islets was examined. With isolated islets from 4- to 12-wk-old NOD mice, a prospective polymerase chain reaction analysis with 18 V beta-specific oligonucleotide primers was performed on the noncloned and unexpanded islet-infiltrating T cells. The methodology used permitted the detection of a minimum of 50 T cells. In contrast to the restricted TCR V beta gene usage reported for other autoimmune diseases, infiltrates of even the youngest mice were characterized by expression of multiple V beta gene segments. |
