| First Author | Grossman A | Year | 1996 |
| Journal | Genomics | Volume | 37 |
| Issue | 2 | Pages | 229-33 |
| PubMed ID | 8921401 | Mgi Jnum | J:36670 |
| Mgi Id | MGI:84097 | Doi | 10.1006/geno.1996.0547 |
| Citation | Grossman A, et al. (1996) Cloning of human lymphocyte-specific interferon regulatory factor (hLSIRF/hIRF4) and mapping of the gene to 6p23-p25. Genomics 37(2):229-33 |
| abstractText | The interferon regulatory factor (IRF) genes encode a family of transcription factors involved in the transcriptional regulation of interferon and the interferon stimulated genes through recognition of the interferon stimulated response element. We previously reported the cloning of a murine lymphocyte-specific IRF (mLSIRF), which was rapidly induced following B- or T-cell receptor crosslinking, To study the role of LSIRF in human lymphocyte development, we have cloned the complete 5.3-kb cDNA for the human homolog (hLSIRF). hLSIRF is a protein of 450 amino acids with a predicted molecular weight of 51.6 kDa and possesses 92% identity at the amino acid level to mLSIRF, including near identity in the DNA- binding domain. In Northern blot analysis, a single transcript of similar to 5 kb was highly expressed in spleen and peripheral blood lymphocyte, hLSIRF mRNA was rapidly induced in peripheral T cells after crosslinking the T-cell receptor, Analysis of tumor cell lines showed that hLSIRF mRNA was basally expressed in most B- but not T-cell lines, Surprisingly hLSIRF mRNA was also found in the melanoma line G361 and is expressed in normal melanocytes as well, Sequence hom a genomic clone for hLSIRF was compared to that from mouse and revealed an identical exon-intron structure and a conserved PU.1- binding motif in the promoter, By FISH analysis, hLSIRF was mapped to 6p23-p25. (C) 1996 Academic Press, Inc. |
