Primary Identifier | MGI:7645419 | Attribute String | Inserted expressed sequence, Null/knockout |
Gene | Trac | Strain of Origin | C57BL/6J |
Is Recombinase | false | Is Wild Type | false |
molecularNote | CRISPR technology was used to introduce an in-frame, low affinity (clone 7431), codon-optimized, Msln406-414:H-2Db-specific Valpha4/Vbeta9 T cell receptor (TCR) clone 7431 consisting of TCRb variable (V), TCRb constant (C) and TCRa V, linked by a self-cleaving P2A element to exon 1 of the mouse Trac gene and simultaneously delete 23 bp from the 5' end of exon 4 of the endogenous Msln gene to create a knock-out allele. |