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Allele : Trac<em2(Tcrb,Tcra)Strom> T cell receptor alpha constant; endonuclease-mediated mutation 2, Ingunn Stromnes

Primary Identifier  MGI:7645419 Attribute String  Inserted expressed sequence, Null/knockout
Gene  Trac Strain of Origin  C57BL/6J
Is Recombinase  false Is Wild Type  false
molecularNote  CRISPR technology was used to introduce an in-frame, low affinity (clone 7431), codon-optimized, Msln406-414:H-2Db-specific Valpha4/Vbeta9 T cell receptor (TCR) clone 7431 consisting of TCRb variable (V), TCRb constant (C) and TCRa V, linked by a self-cleaving P2A element to exon 1 of the mouse Trac gene and simultaneously delete 23 bp from the 5' end of exon 4 of the endogenous Msln gene to create a knock-out allele.
  • mutations:
  • Intragenic deletion,
  • Deletion
  • synonyms:
  • 7431 TRex,
  • 7431 TRex
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1 Feature

Trail: Allele

Genome

2 Expresses

Trail: Allele

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

Trail: Allele

0 Driven By

2 Publication categories

Trail: Allele