| First Author | Schlake T | Year | 1999 |
| Journal | Oncogene | Volume | 18 |
| Issue | 44 | Pages | 6078-82 |
| PubMed ID | 10557097 | Mgi Jnum | J:58364 |
| Mgi Id | MGI:1347410 | Doi | 10.1038/sj.onc.1203021 |
| Citation | Schlake T, et al. (1999) Predetermined chromosomal deletion encompassing the Nf-1 gene. Oncogene 18(44):6078-82 |
| abstractText | Complex chromosomal rearrangements (deletions, inversions, translocations) are a hallmark of human tumour cells. Yet, the generation of animal models for gross chromosomal abnormalities still presents a formidable challenge. Here, we describe a versatile procedure for chromosomal engineering that was used to generate an ES cell line with a megabase deletion encompassing the tumour suppressor gene neurofibromatosis-1 (Nf-1) on mouse chromosome 11, which is often deleted in tumours of neural crest origin. Homologous recombination into sites flanking Nf-1 was used to introduce artificial sequences (triple-helix, loxP, vector backbone) that can be employed for in vitro recovery of intervening sequences or the generation of in vivo deletions. This strategy may be developed into a scheme by which large chromosomal regions with precisely defined end points may be excised from mammalian cells and reintroduced after suitable in vitro modification. |
