| First Author | Gopal-Srivastava R | Year | 1994 |
| Journal | Nucleic Acids Res | Volume | 22 |
| Issue | 7 | Pages | 1281-6 |
| PubMed ID | 8165144 | Mgi Jnum | J:18395 |
| Mgi Id | MGI:66684 | Doi | 10.1093/nar/22.7.1281 |
| Citation | Gopal-Srivastava R, et al. (1994) Identification of a lens-specific regulatory region (LSR) of the murine alpha B-crystallin gene. Nucleic Acids Res 22(7):1281-6 |
| abstractText | Previous studies have shown that the -661/+44 sequence of the murine alpha B-crystallin gene contains a muscle-preferred enhancer (-426/-257) and can drive the bacterial chloramphenicol acetyltransferase (CAT) gene in the lens, skeletal muscle and heart of transgenic mice. Here we show that transgenic mice carrying a truncated -164/+44 fragment of the alpha B-crystallin gene fused to the CAT gene expressed exclusively in the lens; by contrast mice carrying a -426/+44 fragment of the alpha B gene fused to CAT expressed highly in the lens, skeletal muscle and heart, and slightly in the lung, brain, kidney, spleen and liver. DNase I protection experiments indicated that the -147/-118 sequence is protected by nuclear proteins from alpha TN4-1 lens cell line, but not by nuclear proteins from myotubes of the C2C12 cell line. Site directed mutagenesis of this sequence decreased promoter activity in transiently-transfected lens cells, consistent with this sequence being a lens-specific regulatory region (LSR). We conclude that the -426/-257 enhancer is required for expression in skeletal muscle, heart and possibly other tissues, and that the -164/+44 sequence of the alpha B-crystallin gene is sufficient for expression in the lens of transgenic mice. |
