| First Author | Powis RA | Year | 2016 |
| Journal | J Anat | Volume | 228 |
| Issue | 3 | Pages | 443-51 |
| PubMed ID | 26576026 | Mgi Jnum | J:257101 |
| Mgi Id | MGI:6113730 | Doi | 10.1111/joa.12419 |
| Citation | Powis RA, et al. (2016) Selective loss of alpha motor neurons with sparing of gamma motor neurons and spinal cord cholinergic neurons in a mouse model of spinal muscular atrophy. J Anat 228(3):443-51 |
| abstractText | Spinal muscular atrophy (SMA) is a neuromuscular disease characterised primarily by loss of lower motor neurons from the ventral grey horn of the spinal cord and proximal muscle atrophy. Recent experiments utilising mouse models of SMA have demonstrated that not all motor neurons are equally susceptible to the disease, revealing that other populations of neurons can also be affected. Here, we have extended investigations of selective vulnerability of neuronal populations in the spinal cord of SMA mice to include comparative assessments of alpha motor neuron (alpha-MN) and gamma motor neuron (gamma-MN) pools, as well as other populations of cholinergic neurons. Immunohistochemical analyses of late-symptomatic SMA mouse spinal cord revealed that numbers of alpha-MNs were significantly reduced at all levels of the spinal cord compared with controls, whereas numbers of gamma-MNs remained stable. Likewise, the average size of alpha-MN cell somata was decreased in SMA mice with no change occurring in gamma-MNs. Evaluation of other pools of spinal cord cholinergic neurons revealed that pre-ganglionic sympathetic neurons, central canal cluster interneurons, partition interneurons and preganglionic autonomic dorsal commissural nucleus neuron numbers all remained unaffected in SMA mice. Taken together, these findings indicate that alpha-MNs are uniquely vulnerable among cholinergic neuron populations in the SMA mouse spinal cord, with gamma-MNs and other cholinergic neuronal populations being largely spared. |
