| Primary Identifier | MGI:5584100 | Allele Type | Transgenic |
| Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Tg(CAG-RFP*,-Dscam,-EGFP)1Pfu |
| Strain of Origin | (C57BL/6 x SJL)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | This transgene contains (from 5' to 3') the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from the pCAGGS vector), a loxP site, the cytoplasmic red fluorescent protein tdimer2(12) (a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), a polyadenylation signal, a second loxP site, a full-length mouse Down syndrome cell adhesion molecule (Dscam) sequence including the endogenous stop codon, an internal ribosome entry site (IRES), an enhanced green fluorescent protein sequence and a polyadenylation signal. Red fluorescence protein is expressed in all cells/tissues examined. Cre recombinase exposure deletes the floxed tdimer2(12) sequence and results in expression of full-length Dscam and EGFP fluorescence. |
