| Primary Identifier | MGI:7703104 | Allele Type | Endonuclease-mediated |
| Attribute String | Conditional ready, Epitope tag, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Using CRISPR/Cas9 technology, a cassette encoding a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG) followed by (from 5' to 3') a floxed STOP cassette and a 3xHA epitope C-terminal tagged T-box 2 (Tbx2) cDNA, a viral 2A oligopeptide (P2A) self-cleaving peptide, followed by a 3xV5 epitope C-terminal tagged IKAROS family zinc finger 2 (Ikzf2) cDNA, a viral 2A oligopeptide (T2A) self-cleaving peptidee and a EGFP sequence and a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) fused to a polyadenylation signal were inserted into the Gt(ROSA)26Sor locus. |
