Primary Identifier | MGI:7574709 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Sv2a |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of exon 3 and a neomycin resistance gene flanked by self-deletion anchor sites, a lox2272 site, a SA-2A-dTomato-polyA cassette in reverse orientation, a second loxP and lox2272 sites were inserted downstream of exon 3 in intron 3. The neomycin resistance gene was self-excised. Cre-mediated recombination either inverted the dTomato cassette and exon 3 using the loxP sites, such that the dTomato cassette is now upstream of exon 3 or inverted just the dTomato cassette using lox2272. Cre-mediated excionsion using loxP deleted exon 3. |