| First Author | Hata S | Year | 1995 |
| Journal | Biochim Biophys Acta | Volume | 1261 |
| Issue | 1 | Pages | 121-5 |
| PubMed ID | 7893747 | Mgi Jnum | J:23741 |
| Mgi Id | MGI:71432 | Doi | 10.1016/0167-4781(95)00002-x |
| Citation | Hata S, et al. (1995) cDNA cloning of a putative G protein-coupled receptor from brain. Biochim Biophys Acta 1261(1):121-5 |
| abstractText | Using degenerate oligonucleotide primers corresponding to conserved regions of the G-protein coupled receptor superfamily, we carried out a low-stringency polymerase chain reaction and obtained two novel partial-length clones from a rat brain cDNA library. We used one of these clones for conventional library screening and isolated a longer cDNA clone, designated as RBU-15, from another rat brain library. Although RBU-15 was truncated at its 5' end, Northern blot analysis revealed that the gene was expressed in the brain and spleen. Next, we isolated a full-length cDNA clone, designated as HB-954, from a human fetal brain library, using RBU-15 as a probe. The deduced amino acid sequence of HB-954 contained four putative glycosylation sites in the N-terminal part, seven transmembrane domains, and a large cytosolic domain in the C-terminal part. The protein products of RBU-15 and HB-954 likely belong to a distinctive subfamily, because no receptors in the superfamily were found to be closely related to them. |
