| Primary Identifier | MGI:2183491 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mbd4 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A cassette, containing a splice acceptor site and IRES-Beta-geo, was inserted into intron 1 of the endogenous locus. Northern blot and RT-PCR analysis showed levels of transcript in homozygous mutant mice to be less than 0.1% of those in wild-type mice. Two truncated transcripts were identified; one which truncated at the transgenic polyadenylation signal and another in which a frameshift mutation was introduced by the excision of the IRES-Beta-geo transgenic region (leaving the the splice acceptor site in between exons 1 and 2). |
