| First Author | Peng Y | Year | 2009 |
| Journal | J Immunol | Volume | 182 |
| Issue | 5 | Pages | 2777-85 |
| PubMed ID | 19234172 | Mgi Jnum | J:274539 |
| Mgi Id | MGI:6295308 | Doi | 10.4049/jimmunol.0803172 |
| Citation | Peng Y, et al. (2009) Ly6C(low) monocytes differentiate into dendritic cells and cross-tolerize T cells through PDL-1. J Immunol 182(5):2777-85 |
| abstractText | Monocyte-derived dendritic cells are active participants during the immune response against infection, but whether they play a role in maintaining self-tolerance under steady-state conditions is not known. Here we investigated the differentiation of monocytes, their ability to ingest apoptotic cells, and their potential functionality in vivo. We observed that Ly6C (Gr-1)(low) mature monocytes up-regulate their MHC II level in the spleen, express high levels of PDL-1 (programmed death ligand 1), and are more efficient than Ly6C(high) immature monocytes in the ingestion of apoptotic cells in vivo. Sorted circulating Ly6C(low) monocytes were able to cross-present both apoptotic cell-associated OVA and soluble OVA protein. Monocytes containing apoptotic cells can further differentiate into CD11c(+)CD8alpha(-)MHC II(+) splenic dendritic cells that maintained high expression of PDL-1. Since wild-type but not PDL-1-deficient peripheral blood monocytes containing apoptotic cell-associated OVA suppressed the response to OVA immunization, PDL-1 expression was required for monocyte-mediated T cell tolerance. These observations demonstrate that Ly6C(low) mature monocytes can promote tolerance to self Ag contained in apoptotic cells through a PDL-1-dependent mechanism. |
