Primary Identifier | MGI:6367783 | Allele Type | Targeted |
Attribute String | Not Specified | Gene | Prph2 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Two point mutations were introduced to exon 2 of the gene. The first was a silent G>A to eliminate a Hha1 restriction site to aid in genotyping, and the second was the A>G missense mutation to produce the p.N229S substitution in the encoded protein. An FRT site flanked neomycin selection cassette was removed via flp-mediated recombination. |