| First Author | Lu S | Year | 2008 |
| Journal | Cell Motil Cytoskeleton | Volume | 65 |
| Issue | 12 | Pages | 945-54 |
| PubMed ID | 18792955 | Mgi Jnum | J:142599 |
| Mgi Id | MGI:3821810 | Doi | 10.1002/cm.20317 |
| Citation | Lu S, et al. (2008) Expression and alternative splicing of N-RAP during mouse skeletal muscle development. Cell Motil Cytoskeleton 65(12):945-54 |
| abstractText | N-RAP alternative splicing and protein localization were studied in developing skeletal muscle tissue from pre- and postnatal mice and in fusing primary myotubes in culture. Messages encoding N-RAP-s and N-RAP-c, the predominant isoforms of N-RAP detected in adult skeletal muscle and heart, respectively, were present in a 5:1 ratio in skeletal muscle isolated from E16.5 embryos. N-RAP-s mRNA levels increased three-fold over the first 3 weeks of postnatal development, while N-RAP-c mRNA levels remained low. N-RAP alternative splicing during myotube differentiation in culture was similar to the pattern observed in embryonic and neonatal muscle, with N-RAP-s expression increasing and N-RAP-c mRNA levels remaining low. In both developing skeletal muscle and cultured myotubes, N-RAP protein was primarily associated with developing myofibrillar structures containing alpha-actinin, but was not present in mature myofibrils. The results establish that N-RAP-s is the predominant spliced form of N-RAP present throughout skeletal muscle development. |
