| Type | MGI:General | Description | after 12 hours of treatment with 200 ng/ml nocodazole (induces spindle damage) only 2.5% of heterozygous MEFs are arrested compared to 15% of wild-type cells and after 4 hours in serum without nocodazole (recovery phase) more cells are present with 4N DNA content suggesting progression to G1 without cell division overexpression of Rae1 can rescue the mitotic checkpoint defect |
