| Primary Identifier | MGI:5562871 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Adcyap1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | Using recombineering methods, a selection cassette containing an internal ribosomal entry sequence (IRES) linked to cre and an frt-flanked kanamycin gene was inserted immediately downstream of the stop codon in exon 5 of the Adcyap1 gene in a BAC. This construct was used to isolate a targeting plasmid with the cre-kanamycin cassette with upstream and downstream Adcyap1 homology arms. ES cells were targeted and used for blastocyst injection. Chimeras were bred for germline transmission. The selection marker was excised by breeding to Flp deleter animals. |
