| First Author | Okazaki N | Year | 2008 |
| Journal | J Mol Biol | Volume | 382 |
| Issue | 2 | Pages | 257-65 |
| PubMed ID | 18675275 | Mgi Jnum | J:168969 |
| Mgi Id | MGI:4939352 | Doi | 10.1016/j.jmb.2008.07.031 |
| Citation | Okazaki N, et al. (2008) The novel protein complex with SMARCAD1/KIAA1122 binds to the vicinity of TSS. J Mol Biol 382(2):257-65 |
| abstractText | The SMARCAD1/KIAA1122 protein is structurally classified into the SWI2/SNF2 superfamily of DNA-dependent ATPases that are catalytic subunits of chromatin-remodeling complexes. Although the importance of other members of the SWR1-like subfamily in chromatin remodeling (EP400, INOC1, and SRCAP) has already been elucidated, the biological function of SMARCAD1/KIAA1122 in transcriptional regulation remains to be clarified. To gain insight into the role of this protein, we generated a specific antibody against SMARCAD1/KIAA1122 and used it for chromatin and protein immunoprecipitation assays. We employed high-resolution genome tiling microarrays in chromatin immunoprecipitation and found the binding sites of SMARCAD1/KIAA1122 in the vicinity of the transcriptional start site of 69 candidate target genes. In the protein immunoprecipitation assay, we found that endogenous SMARCAD1/KIAA1122 binds with TRIM28, a recently highlighted transcriptional regulator in the cancer field. From these findings, we propose a novel model for gene regulation via the SMARCAD1/KIAA1122 protein complex. |
