| First Author | Lu LY | Year | 2015 |
| Journal | J Biol Chem | Volume | 290 |
| Issue | 2 | Pages | 851-60 |
| PubMed ID | 25451918 | Mgi Jnum | J:218761 |
| Mgi Id | MGI:5618360 | Doi | 10.1074/jbc.M114.611509 |
| Citation | Lu LY, et al. (2015) Topoisomerase II regulates the maintenance of DNA methylation. J Biol Chem 290(2):851-60 |
| abstractText | The maintenance of DNA methylation in nascent DNA is a critical event for numerous biological processes. Following DNA replication, DNMT1 is the key enzyme that strictly copies the methylation pattern from the parental strand to the nascent DNA. However, the mechanism underlying this highly specific event is not thoroughly understood. In this study, we identified topoisomerase IIalpha (TopoIIalpha) as a novel regulator of the maintenance DNA methylation. UHRF1, a protein important for global DNA methylation, interacts with TopoIIalpha and regulates its localization to hemimethylated DNA. TopoIIalpha decatenates the hemimethylated DNA following replication, which might facilitate the methylation of the nascent strand by DNMT1. Inhibiting this activity impairs DNA methylation at multiple genomic loci. We have uncovered a novel mechanism during the maintenance of DNA methylation. |
